Chapter 20A Homework Questions

1. Describe the basic process in cloning a gene.

2. What are restriction enzymes? What is there normal function in the cell?

3. What is the average DNA chain length of a restriction enzyme that recognizes 4 bases? 6 bases?

4. E. coli strains that are used for genetic engineering must be what with respect to recombination? Explain?

5. What genetic elements must all cloning vectors have?

6. Describe several methods for identify a gene of interest in a cloning experiment?

7. What is a DNA library?

8. What process is in vitro DNA amplification?

9. Describe Southern blotting?

10. What is a restriction fragment length polymorphism?