Chapter 20B Homework Questions

1. Describe the polymerase chain reaction.

2. What is a yeast artificial chromosome? What genetic elements must it have?

3. What is cDNA and how is it made?

4. Compare a Southern blot with a Northern blot and a Western blot?

5. Describe how DNA is sequenced?

6. How can RFLP’s be used in forensics and paternity questions? Use a diagram.

7. Name ten organisms which have had their genomes sequenced? Make a table of their genome size, number of genes, number of chromosomes/

8. What process is in vitro DNA amplification?

9. Describe “shotgun” sequencing.

10. What structure is used to identify a presumptive gene in a sequence of DNA?