Virtual Fly Lab

	Virtual Fly Lab
	Introduction
	In this exercise you will construct a series of crosses of Drosophila melanogaster using the following web site: http://www.sciencecourseware.com/vcise/drosophila/ Log into this web site as a guest and follow the instructions to construct the specified crosses listed below. Record the results from each cross and incorporate the data into your lab report. Use the following sites: http://www.ndsu.edu/pubweb/~mcclean/plsc431/mendel/mendel4.htm http://www.colby.edu/biology/BI17x/freq.html http://math.hws.edu/javamath/ryan/ChiSquare.html http://faculty.chass.ncsu.edu/garson/PA765/chisq.htm to conduct the chi-square statistical analysis of each of your crosses.
	Crosses:
	1. Select a non-sex-linked gene (refer to the Drosophila genetic map provided under handouts) and perform a monohybrid cross. Construct a Punnett square of your expected probabilities for your chi square analysis.

	2. Select two non-sex-linked genes and perform a dihybrid cross. Construct a Punnett square of your expected probabilities for your chi square analysis.
	3. Perform a cross between a black bodied, purple eyed female and a wild type (red eye/yellow striped body) male. What is the phenotype of the F1 generation. Cross the F1 males to the black bodied/purple eyed female (a test cross). Construct a Punnett square to show your expected results. Compare your expected results to the results that you obtain. Perform a chi-square analysis. What most likely accounts for the results?
	4. Perform a cross between female miniature winged, tan body female and a wild type male. Construct a Punnett square of your expected probabilities for your chi square analysis. Use the females from this cross (which should be of what geneotype?) and cross them to a white eyed male.
	5. Examine the genetic map under handouts. Select three autosomal non-sex-linked genes that are on the same chromosome (linked genes) and that have mutants available in the fly lab. Perform a three point test cross by first crossing the triple mutant to the wild type and then back crossing the heterozygous F1 to the homozygous recessive. Are the results consistant with the linkage map? Use the following for more information: http://biology.clc.uc.edu/Courses/bio105/sex-link.htm http://www.animalgenome.org/edu/QTL/Julius_notes/05_linkagemap.PDF http://www.life.illinois.edu/ib/201/lectures/Mapping.pdf
	Extra Credit: Construct a sex-dihybrid cross to map two genes on the X- or number one chromosome of Drosophila.

	Extra Extra Credit: Write a two page biography of Thomas Hunt Morgan with references. (Cannot be cut and pasted from web sites-must be your own work.