Instructions for the Transformation and DNA Fingerprinting Lab Report, Experiment #6

Refer to following links:

Transformation Student Manual: PGLO Student Manual

DNA Fingerprinting Student Manual: DNA Fingerprint Student Manual

With respect to the transformation lab do the following:

a. Summarize the experiment in your own words.

b. Make a table of the results.

c. Answer the following questions:

1. What was the purpose of the calcium chloride transformation solution?

2. Why was arabinose included in the media of one of the positive DNA plates?

3. What was required to select for the growth of bacteria that had been transformed?

4. Based on the above what could you use to select for transformants in higher organisms like yeast, fruit flies, mice and human beings?

5. What do you think might happen if glucose were added to the arabinose containing plate?

6. Describe an experiment or series of experiments in which you would investigate transformation efficiency (# of transformants/ug of DNA) relative to the concentration and type transforming solution.

7. In this experiment, what was the transformation efficency of your transformation?

8. What genes were used in the first transformation experiment conducted by Herb Boyer, Stanley Cohen and Annie Chang?

9. In this experiment you used a media called Luria broth. Who was Luria?

10. What is the difference between cloning an entire organism and cloning a gene?

With respect to the DNA fingerprinting lab do the following:

a. Summarize the experiment in your own words.

b. Make a drawing or add in a picture of your results Who is the most likely suspect?

c. Answer the following questions:

1. Describe the basis of separation in DNA gel electrophoresis?

2. What would the gel look like if the restriction enzymes had not cut the DNA?

3. Answer the Lesson 4 Questions page 34, Student Manual #1-9.